Journal: Journal of the American Chemical Society

Article Title: Encapsulation of Gold-Based Anticancer Agents in Protease-Degradable Peptide Nanofilaments Enhances Their Potency

doi: 10.1021/jacs.2c09820

Figure Lengend Snippet: Cell Viability of gold anticancer agents, gold-loaded peptide nanofilaments ( PD+1 , PD+2 , AD+1 , AD+2 ), and peptides ( PD , AD ) in three human cell lines. Caki-1 (renal cancer), MDA-MB-231 (Triple Negative Breast Cancer), and IMR-90 (lung fibroblasts) incubated with drug-loaded peptide for 72 h. A) Compound 1 , B) Compound 2 . (* represents P <0.05, ** represents P <0.01, *** represents P <0.001).

Article Snippet: Human breast adenocarcinoma line MDA-MB-231 and human lung fibroblast line IMR-90 were obtained from the American Type Culture Collection (ATCC; Manassas, Virginia, USA) and cultured with Dulbecco’s modified Eagle’s medium (DMEM; Fisher Scientific) containing 10 % fetal bovine serum, certified, heat inactivated, US origin (FBS; Fisher Scientific), 1 % minimum essential media (MEM) nonessential amino acids (NEAA; Fisher Scientific), and 1 % penicillin–streptomycin (PenStrep; Fisher Scientific).

Techniques: Incubation

Journal: Cancers

Article Title: Lidocaine Suppresses Viability and Migration of Human Breast Cancer Cells: TRPM7 as a Target for Some Breast Cancer Cell Lines

doi: 10.3390/cancers13020234

Figure Lengend Snippet: The effect of lidocaine on TRPM7 channels in HEK cells. ( A ). Patch-clamp assay. WT-HEK cells were used as a negative control for the cell model (black). 2-APB (200 µM) was used as a positive control (gray). [lidocaine] ≥ 1 mM suppressed TRPM7-like current in HEK-M7, n = 6, “*” indicates the significant differences ( p < 0.05) compared with the control (purple). ( B ). Representative TRPM7-like current from patch-clamp. ( C ). Fluorescence quench assay: [lidocaine] ≥ 0.3 mM concentration-dependently decreased the influx of Mn 2+ in HEK-M7 cells ( n = 3). Current and quench data of control, negative control, and 200 µM 2-APB were published previously .

Article Snippet: TRPM7 knock-out MDA-MB-231 cell line (KO-231) was obtained from GenScript (Piscataway, NJ, USA) through GenCRISPRTM Technology [ ].

Techniques: Patch Clamp, Negative Control, Positive Control, Control, Fluorescence, Concentration Assay

Journal: Cancers

Article Title: Lidocaine Suppresses Viability and Migration of Human Breast Cancer Cells: TRPM7 as a Target for Some Breast Cancer Cell Lines

doi: 10.3390/cancers13020234

Figure Lengend Snippet: The effect of lidocaine on TRPM7 channels in breast cancer cell lines. ( A – D ). Fluorescence quench assay ( n = 3): The fluorescence quenches followed by the addition of Mn 2+ were plotted on the same scale. 10 µM Gd 2+ was used as the negative control. ( E – H ). The average fluorescence quench amount of 200–300 s in MDA-MB-231, AU565, T47D, and MDA-MB-468. “*” indicates the significant differences ( p < 0.05) compared with the control. The quench data of the vehicle, negative, and 200 µ 2-APB in MDA-MB-231 were published previously .

Article Snippet: TRPM7 knock-out MDA-MB-231 cell line (KO-231) was obtained from GenScript (Piscataway, NJ, USA) through GenCRISPRTM Technology [ ].

Techniques: Fluorescence, Negative Control, Control

Journal: Cancers

Article Title: Lidocaine Suppresses Viability and Migration of Human Breast Cancer Cells: TRPM7 as a Target for Some Breast Cancer Cell Lines

doi: 10.3390/cancers13020234

Figure Lengend Snippet: Correlations among the effects of 1 mM lidocaine on TRPM7 function, cell viability, and cell migration. ( A ). Suppression of migration vs. viability in 7 breast cancer cell lines and HEK-M7 cells (slope = 0.7954, r 2 = 0.4016). ( B ). Suppression of viability vs. inhibition of TRPM7 function for HEK-M7 cells and the four breast cancer cells showing significant changes in fluorescence in the flux quenching assay (slope = 0.7426, r 2 = 0.9351). ( C ). Suppression of migration vs. inhibition of TRPM7 function for HEK-M7 cells and the four breast cancer cells showing significant changes in fluorescence in the flux quenching assay (slope = 0.7563, r 2 = 0.7047). TRPM7 function inhibition was calculated from the average of the quench fluorescence during the last 30 s. Linear regression was plotted and the 95% confidence band of the best fit line was shown.

Article Snippet: TRPM7 knock-out MDA-MB-231 cell line (KO-231) was obtained from GenScript (Piscataway, NJ, USA) through GenCRISPRTM Technology [ ].

Techniques: Migration, Inhibition, Fluorescence

Journal: Cancers

Article Title: Lidocaine Suppresses Viability and Migration of Human Breast Cancer Cells: TRPM7 as a Target for Some Breast Cancer Cell Lines

doi: 10.3390/cancers13020234

Figure Lengend Snippet: TRPM7 is necessary for the suppression of viability and migration by 0.3 and 1 mM lidocaine. Viability ( A ) and migration ( B ) of HEK cells containing very low levels of TRPM7 (WT-HEK) are unaffected by low concentrations of lidocaine. In contrast, viability and migration of HEK-M7 cells, in which TRPM7 is overexpressed, are suppressed. Viability ( C ) and migration ( D ) of MDA-MB-231 cells in which TRPM7 has been knocked out (KO-231) are not affected by low concentrations of lidocaine. In contrast, viability and migration of the wild-type cell line (WT-231, which express TRPM7) are suppressed. “*” indicates the significant differences ( p < 0.05).

Article Snippet: TRPM7 knock-out MDA-MB-231 cell line (KO-231) was obtained from GenScript (Piscataway, NJ, USA) through GenCRISPRTM Technology [ ].

Techniques: Migration